Archive for June, 2009

Curcumin metabolites; production in liver cells and live rats and activity against cyclooxygenase-2

Saturday, June 6th, 2009

Curcumin is the chemical part of tumeric which is most active. It prevents cancer in rodents, particularly in the intestines. However curcumin is poorly absorbed when given by mouth. Very little gets into the bloodstream. So these researchers wondered if metabolites of curcumin where really causing the beneficial affects attributed to curcumin. Metabolites are the products made through the metabolism of a chemical in the body. First, they found that isolated liver cells of rats and humans produced two metabolites, hexahydrocurcumin and hexahydrocurcuminol. Rats given curcumin by injection mostly made curcumin glucuronide and curcumin sulfate. They made little hexahydrocurcumin, hexahydrocurcuminol, and hexahydrocurcumin glucuronide. They then studied the effect of these metabolites on an enzyme important in the prevention of cancer, cyclooxygenase-2 (COX-2). They compared how much four metabolites blocked COX-2 activity in cells from the surface the human large intestine. Curcumin complete inhibited COX-2. Tetrahydrocurcumin, hexahydrocurcumin, and curcumin sulfate, only weakly inhibited COX-s. Tetrahydrocurcumin has been shown to be metabolite of curcumin in mice. Hexahydrocurcuminol had no effect. These scientists concluded that the live rats made different curcumin metabolites than did liver cells in a test tube. The metabolites studied inhibit COX-2 had less than curcumin. When curcumin is given by mouth, the cells on the surface of the intestine are directly exposed to curcumin, not metabolites. Therefore they advise studying curcumin as a treatment in colon and rectal cancer.

Characterization of metabolites of the chemopreven…[Cancer Res. 2001]

Rats fed curcumin reduced experimental cataracts

Saturday, June 6th, 2009

Naphthalene is known to cause cataracts (clouding over of the lens of the eye) in rats and rabbits. Therefore experimental animals are treated with naphthalene in order to model and study cataracts of elderly humans. It is thought that naphthalene causes cataracts through oxidative stress. Curcumin is the most active chemical part of turmeric, a spice used in Indian curry dishes. It is an effective antioxidant. These scientists studied whether low amounts of dietary curcumin slowed the formation of the cataracts usually caused by naphthalene in rats. They looked for apoptotic cells in the cells covering the lens by measuring DNA formation. Both treated rats and control rats were given naphalene to produce experimental cataracts. Only 0.005% (w/w) curcumin was added to the diet of the treated rats. The treated rats had significantly less clouding of their lenses as compared to the control rats. They found, for the first time, that cataracts caused by naphthalene involved apoptosis of the cells covering the lens. Apoptosis is a type of programmed, deliberate cell death in which the cell corpses and fragments are safely disposed. They also found that that curcumin reduced the apoptotic effect of naphthalene.

Tumeric extract by mouth in rabbits reduces the fats that lead to heart disease.

Saturday, June 6th, 2009

Curcumin is a yellow pigment obtained from roots of Curcuma longa commonly used as a spice and food colouring. These researchers looked at whether curcumin affected the fats that lead to atherosclerosis. They evaluated the effect of an alcohol-water extract obtained from rhizomes of C. longa on these fats in rabbits. To cause atherosclerosis the 18 study rabbits were fed a diet of 95.7% standard chow, 3% lard and 1. 3% cholesterol for 7 weeks. The rabbits were divided into three groups, two treatment groups and a control group. The two treatments were given turmeric extract by mouth, either 1.66 or 3.2 milligrams per kilogram of body weight. The level of several fats and related chemicals and enzymes in the plasma, the liquid part of the blood, was measured. These included low density lipoprotein (LDL) lipid structure, alpha-tocopherol, retinol, LDL thiobarbiturate reactive substances, and LDL lipid hydroperoxides. The concentration of the thiobarbiturate reactive substances is an index of lipid peroxidation and oxidative stress. They also examined the wounds atherosclerotic in the large blood vessel, the aorta. Only the low dose of the extract decreases the peroxidation of LDL. Both treatment groups had lower levels of total plasma cholesterol than the control group. The rabbits treated with the lower dose had lower levels of cholesterol, phospholipids and triglycerides in LDL than the animals treated with the higher dose. They concluded that this extract could be useful in treating diseases of the heart and blood vessels caused by atherosclerosis

Oral administration of a turmeric extract inhibits…[Atherosclerosis. 1999]

Effect of tumeric and curcumin on the chromosomal damage caused by radiation

Saturday, June 6th, 2009

Both the Indian spice tumeric and curcumin (the most active chemical part of tumeric) are anti-oxidants. Anti-oxidants, chemicals that reduce free radicals, are widely used to try to prevent cancer. These investigators look at whether tumeric and curcumin changed the amount of chromosome damage caused by gamma radiation. Chinese hamster ovary cells were treated with three doses of each drug. The doses were; turmeric (100, 250, and 500 micrograms per milliliter) and curcumin (2.5, 5, and 10 micrograms per milliliter). Then they exposed the cells to radiation (2.5 grays) during different phases of the cell cycle. By itself, turmeric did not increase chromosomal damage. In contrast, curcumin, at 10 micrograms per milliliter, increased the frequency of chromosomal damage. Neither treatment protected the cells from the chromosomal damage caused by radiation. Instead, they found an obvious increase in chromosome abnormalities when two of the doses were combined with radiation. The abnormalities were increased when cells were treated with 500 microg/ml turmeric and radiation during G2/S phase of the cell cycle. The chromosomal abnormalities were also increased with 10 microg/ml curcumin plus radiation during S and G2/S phases. These are the parts of the cycle when DNA is replicated and the cell is prepared for division. The results clearly showed that tumeric and curcumin worsened the damage caused by radiation. They suggest that these treatments can boost the harmful effects of radiation depending on the experimental conditions

Potentiation by turmeric and curcumin of gamma-rad…[Teratog Carcinog Mutagen. 1999]

Kidney disease in diabetic rats was improved by curcumin in the diet

Saturday, June 6th, 2009

Curcumin is the most active part the yellow Indian spice turmeric (Curcuma longa). These scientists studied its effect in kidney disease in rats. The rats served as a model of the kidney disease that occurs in humans with diabetes. The chemical streptozotocin was used to cause diabetes in Wistar rats. Then the rats were fed Curcumin (0.5%) for 8 weeks. Kidney damage was measured by the amount of proteins passed in the urine. Healthy kidneys do not pass proteins in the urine. They also looked at whether four enzymes that are part of the kidney leaked out into the urine. They measured activities of several key kidney enzymes. These included glucose-6-phosphate dehydrogenase, glucose-6-phosphatase, lactate dehydrogenase, aldose reductase, sorbitol dehydrogenase transaminases, and ATPases. They measured the ratio of polyunsaturated fatty acids to saturated fatty acids. This ratio reflects the health of the kidney membranes. Some of their results showed that curcumin lead to significant reduction in the kidney disease caused by diabetes in these rats. Beneficial conclusions were based on the amount of enzymes and protein in the urine, the activity of kidney ATPases and fatty acid composition of renal membranes. These findings agreed with what they saw when they looked at slices of the kidneys under the microscope. They believe that the improvements they found may have been due to curcumins lowering of blood cholesterol levels.

Amelioration of renal lesions associated with diab…[Mol Cell Biochem. 1998]